Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under …Product Description. Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase (HRP) for chemiluminescent detection. This product is thoroughly validated with CST primary antibodies and will work optimally with the CST ...Cell signaling is a fundamental property of all cellular life in prokaryotes and eukaryotes . Typically, the signaling process involves three components: the signal, the receptor, and the effector. [citation needed] In biology, signals are mostly chemical in nature, but can also be physical cues such as pressure, voltage, temperature, or light.Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected] using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Hr -Horse. -Guinea Pig. -Rabbit. -All Species Expected. Monoclonal Antibody for studying NeuN. Cited in 47 publications. Validated for WB, IP, IF. Highly specific and rigorously validated in-house, NeuN (E4M5P) Mouse Monoclonal Antibody (CST … The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3).Immunoprecipitation of NF-kB p65 from CHO cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is NF-κB p65 (D14E12) XP ® Rabbit mAb, #8242. Western blot was performed using NF-κB p65 (L8F6) Mouse mAb, #6956.Science & Development. As a company of scientists for scientists, we are passionate about the discovery and development of innovative therapies for human disease. Help advance …A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ...Product Description. The Senescence β-Galactosidase Staining Kit is designed to conveniently provide reagents needed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells. Papers have published using this kit in both cells and frozen tissue. The kit includes all reagents necessary for this assay.Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Montminy, M. (1997) Annu Rev Biochem 66, 807-22. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, …Ferroptosis is an iron-dependent form of regulated cell death associated with an increase in lipid peroxides (reviewed in 1,2). Free divalent iron (Fe2+) can lead to spontaneous lipid peroxidation through a Fenton reaction. Ferroptosis is regulated by signaling pathways that control iron storage and oxidative stress.CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and …Apr 15, 2022 · Cell Signaling Technology (CST) emphasizes that it was founded by scientists, for scientists. This is at the core of the company’s culture and permeates all its decisions. Headed by neuroscientist Michael Comb, CST seeks to deeply understand the requirements of their customers—bench researchers who are in the weeds of complex research ... At Cell Signaling Technology (CST), our goal is to provide highly specific antibodies that yield strong, specific signal with minimal background. Our scientists screen a large number of antibodies and recommend only those best suited for the application. Our validation efforts include extensive protocol optimization and antibody titrationAt Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.Western blot analysis of extracts from control HeLa cells (lane 1) or Vimentin knockout HeLa cells (lane 2) using Vimentin (D21H3) XP ® Rabbit mAb #5741 (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Show More.Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic ...Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for ...If you're looking for a career that you can feel good about with a company that cares about you, the environment, and our communities, look no further than Cell Signaling Technology. Check out our current career … Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody. Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using c-Myc Antibody #9402.SignalStar Multiplex IHC from CST is a revolutionary new tool for spatial biology research, providing fully flexible and highly-validated antibody panels. SignalStar technology amplifies multiple biomarkers simultaneously in FFPE tissue with high sensitivity and specificity. Accurate, reliable data can be generated on up to 8 targets in just 2 ...We would like to show you a description here but the site won’t allow us.Pathways and Diagrams. Quickly identify your next research target using the interactive pathways, diagrams, guides and cellular landscapes in each of these collections. These resources provide insights into relevant cellular processes, structures and related diseases to help you identify related proteins and cellular mechanisms to investigate ...At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.Directions for Use: Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).Videos & Webinars | Cell Signaling Technology. USA. Technical Support +1-877-678-8324. Contact Us. PhosphoSitePlus ®. Register Sign In. Quick Add.Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat.Background. Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the ...Species Cross-Reactivity Key: Polyclonal Antibody for studying DRP1 (Ser616) phosphate. Cited in 343 publications. Validated for WB, IP, IF, F. Highly specific and rigorously validated in-house, Phospho-DRP1 (Ser616) Antibody (CST #3455) is ready to ship.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality ...This is true whether the cell is a single-celled organism or part of a larger, more complex multicellular organism. Cells communicate with their environment through a process called signaling. Cell signaling is how the cell collects information and then responds with an action at the correct time. Signaling is the initial event associated with ...Product Description. This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is ...A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ...Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on … Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is β3-Tubulin (D71G9) XP ® Rabbit mAb. Western blot analysis was performed using β3-Tubulin (D71G9) XP ® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody. Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.Immunoprecipitation of ATF-6 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis was performed using ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin (1 mM ...Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...The LC3B (D11) XP ® Rabbit mAb confirms silencing of LC3B expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of LC3B siRNA. Show More. Simple Western™ analysis of lysates (1mg/ml) from HeLa cells treated with Chloroquine (50uM, O/N) using LC3B (D11) XP ® Rabbit mAb #3868.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and … Prior to Cell Signaling Technology, Dana held various IT management positions at Ironwood Pharmaceuticals and Biogen, supporting a wide variety of verticals, including Quality, Manufacturing, Supply Chain, Regulatory, Clinical, Drug Safety, HR, Legal and Finance. She has extensive experience implementing and maintaining LIMS, SDMS, QMS, and ERP ... Western blot analysis of extracts from control HeLa cells (lane 1) or Vimentin knockout HeLa cells (lane 2) using Vimentin (D21H3) XP ® Rabbit mAb #5741 (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Show More. Rabbit IgG. Monoclonal Antibody for studying LAMP1. Cited in 477 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP1 (D2D11) XP® Rabbit Monoclonal Antibody (CST #9091) is ready to ship. At Cell Signaling Technology (CST), we have developed a panel of detection reagents that are designed to recognize a broad range of CARs: Anti-CAR linker antibodies. These first-to-market reagents can be incorporated into multiparametric flow panels for monitoring CAR expression, trafficking, and persistence in preclinical models. Immunoprecipitation of STING from HL-60 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or STING (D2P2F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STING (D2P2F) Rabbit mAb. At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is β3-Tubulin (D71G9) XP ® Rabbit mAb. Western blot analysis was performed using β3-Tubulin (D71G9) XP ® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody. Immunoprecipitation of TIM-3 from RPMI 8226 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is TIM-3 (D5D5R ™) XP ® Rabbit mAb, #45208. Western blot was performed using TIM-3 (D5D5R ™) XP ® Rabbit mAb.Cell Signaling Technology has an overall rating of 4.5 out of 5, based on over 132 reviews left anonymously by employees. 96% of employees would recommend working at Cell Signaling Technology to a friend and 96% have a positive outlook for the business. This rating has improved by 2% over the last 12 months.Western blot analysis of extracts from various cell lines using Alix (E6P9B) Rabbit mAb. Immunoprecipitation of Alix from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control, and lane 3 is Alix (E6P9B) Rabbit mAb. Western blot was performed using Alix (E6P9B) Rabbit mAb. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). Protein extracts from 3T3-Src cells were profiled by PhosphoScan ® to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified (5). Cantley, L.C. (2002) Science 296, 1655-7. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under …Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9). Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 and TCRβ . While you know that each protein is found at the plasma membrane, you need to check whether the antibodies recognize extracellular or intracellular portions of each. Cell Signaling Technology (CST) is dedicated to providing the world’s highest quality, innovative research products to accelerate biological understanding. A privately …Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411.Western blot analysis was performed using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. Confocal immunofluorescent analysis of HeLa cells, serum starved (left) or TNF-α treated (#8902 at 20 ng/ml for 20 min, right), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb ...Mar 14, 2023 · A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ... Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).At Cell Signaling Technology (CST), our goal is to provide highly specific antibodies that yield strong, specific signal with minimal background. Our scientists screen a large number of antibodies and recommend only those best suited for the application. Our validation efforts include extensive protocol optimization and antibody titrationCell Signaling presents the principles and components that underlie all known signaling processes. It provides undergraduate and graduate students the conceptual tools needed …Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas. Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. Show More. Background. Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the ...Wash sections in wash buffer for 5 min. Block each section with 100–400 µl of preferred blocking solution for 1 hr at room temperature. Remove blocking solution and add 100–400 µl primary antibody diluted in SignalStain ® Antibody Diluent ( #8112) to each section. Incubate overnight at 4°C.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. At Cell Signaling Technology (CST), we have developed a panel of detection reagents that are designed to recognize a broad range of CARs: Anti-CAR linker antibodies. These first-to-market reagents can be incorporated into multiparametric flow panels for monitoring CAR expression, trafficking, and persistence in preclinical models. p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ...CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an ...The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Tommy nails, West liberty vet, Youth baseba, Jayson home, Bob tom show, Rush funplex, Kittiwat, Wheeling nailers, Lex live, Satanic temple salem, Gunner dog kennel, Jenkinson's boardwalk point pleasant, Craft supply usa, Reaume
Species Cross-Reactivity Key: Polyclonal Antibody for studying eNOS. Cited in 167 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, eNOS Antibody (CST #9572) is ready to ship.. Genesee theatre waukegan il
Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic ...Apr 15, 2022 · Cell Signaling Technology (CST) emphasizes that it was founded by scientists, for scientists. This is at the core of the company’s culture and permeates all its decisions. Headed by neuroscientist Michael Comb, CST seeks to deeply understand the requirements of their customers—bench researchers who are in the weeds of complex research ... Cell Signaling Technology poster included with each book. The two-sided poster details important signaling pathways. Chapter 12, How Cells Make Decisions, offers a unique visual and interrogative approach that … Cell-cell signaling involves the transmission of a signal from a sending cell to a receiving cell. However, not all sending and receiving cells are next-door neighbors, nor do all cell pairs exchange signals in the same way. There are four basic categories of chemical signaling found in multicellular organisms: paracrine signaling, autocrine ... PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates ...Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic ... For reproducible data, Cell Signaling Technology (CST) offers rigorously validated and reliable products including antibodies, reagents, and kits for biological research. We have a substantial portfolio of products to interrogate mechanisms of cellular health and disease. Whether in the fields of cancer, immunology, infectious disease ... IHC-validated Antibodies for Cellular Senescence. Accelerate and increase confidence in your research results with IHC-validated p16 INK4A (BC42). An antibody shouldn’t be one of the variables in your experiment. Find out why customers …At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.At Cell Signaling Technology (CST), we have developed a panel of detection reagents that are designed to recognize a broad range of CARs: Anti-CAR linker antibodies. …The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and Phospho-CREB (Ser133) (87G3) Rabbit mAb, using CUT&RUN Assay Kit #86652.The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody. Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using c-Myc Antibody #9402.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. Background. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ ... Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Confocal immunofluorescent images of HUVEC cells untreated (left) or stimulated with Vascular Endothelial Growth Factor (VEGF) #9943 (right) and labeled with Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (top, green) and VEGF Receptor 2 (55B11) Rabbit mAb (bottom, green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye). Cell Signaling Technology poster included with each book. The two-sided poster details important signaling pathways. Chapter 12, How Cells Make Decisions , offers a unique visual and interrogative approach that explores four key signaling pathways from teh organismal to molecular levels. The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). If you're looking for a career that you can feel good about with a company that cares about you, the environment, and our communities, look no further than Cell Signaling Technology. Check out our current career … Background. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ ... p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ...Background. TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4).If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ... NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2).GPX4 converts lipid hydroperoxides to non-toxic lipid alcohols, therefore preventing ferroptosis (2). Research studies show that selenium enhances GPX4 expression and inhibits ferroptotic death to protect neurons (3). In addition, some therapy-resistant cancer cells depend on GPX4 to …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Species Cross-Reactivity Key: Polyclonal Antibody for studying DRP1 (Ser616) phosphate. Cited in 343 publications. Validated for WB, IP, IF, F. Highly specific and rigorously validated in-house, Phospho-DRP1 (Ser616) Antibody (CST #3455) is ready to ship.Use the Cell Signaling Technology Technical Documentation search tool to find Product Data Sheets, Certificates of Analysis (CoA), and Safety Data Sheets (SDS). Begin your search by entering the product number in the search field. Online publication of this documentation makes it easier for you to access it, and more environmentally friendly ...Directions for Use: Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and …Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.DIVERSITY, EQUITY, & INCLUSION. CST understands the importance of a safe and diverse workplace. Different perspectives and ideas drive collaboration and innovation. By providing an inclusive and stimulating work environment, we aim to establish human dignity and meaningful work as an essential part of our culture. Embrace the. culture.The interactive pathways and diagrams have been created by CST scientists and outside experts to provide concise overviews of selected signaling pathways and cellular mechanisms. Protein nodes in each interactive diagram are linked to specific antibody product information, as well as protein-specific listings in the PhosphoSitePlus ® …Immunoprecipitation of Bax from HepG2 cells. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Bax (D2E11) Rabbit mAb, #5023. Western blot was performed …GPX4 converts lipid hydroperoxides to non-toxic lipid alcohols, therefore preventing ferroptosis (2). Research studies show that selenium enhances GPX4 expression and inhibits ferroptotic death to protect neurons (3). In addition, some therapy-resistant cancer cells depend on GPX4 to …Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411.SignalStar Multiplex IHC from CST is a revolutionary new tool for spatial biology research, providing fully flexible and highly-validated antibody panels. SignalStar technology amplifies multiple biomarkers simultaneously in FFPE tissue with high sensitivity and specificity. Accurate, reliable data can be generated on up to 8 targets in just 2 ...Polyclonal Antibody for studying Flag-Tag. Cited in 774 publications. Validated for WB, IP, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #2368) is … Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is β3-Tubulin (D71G9) XP ® Rabbit mAb. Western blot analysis was performed using β3-Tubulin (D71G9) XP ® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody. XP ® monoclonal antibodies are high quality rabbit monoclonal antibodies exclusively available from Cell Signaling Technology (CST). XP monoclonal antibodies are generated using XMT ® technology, a proprietary rabbit monoclonal method developed at CST. This technology provides access to a broad range of antibodies unattainable with …CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an ... Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411. Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Western blot analysis of extracts from various cell lines using Alix (E6P9B) Rabbit mAb. Immunoprecipitation of Alix from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control, and lane 3 is Alix (E6P9B) Rabbit mAb. Western blot was performed using Alix (E6P9B) Rabbit mAb. For reproducible data, Cell Signaling Technology (CST) offers rigorously validated and reliable products including antibodies, reagents, and kits for biological research. We have a substantial portfolio of products to interrogate mechanisms of cellular health and disease. Whether in the fields of cancer, immunology, infectious disease ... Lanes 1 & 2 are 5% input. Confocal immunofluorescent analysis of NCI-H1299 cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-DRP1 (Ser616) Antibody (green). Actin filaments have been labeled with DY-554 phallodin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).Cell Signaling Technology (CST) a private company founded by scientist, understands your need to make decision with confidence, providing the best-in-class products and services. No vendor is more stringent about antibody validation than CST, so when you partner with us you get results you can trust faster, to move your oncology project forward ...Videos & Webinars | Cell Signaling Technology. USA. Technical Support +1-877-678-8324. Contact Us. PhosphoSitePlus ®. Register Sign In. Quick Add. Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Since 2000, Cell Signaling Technology (CST) has offered a rigorously tested and well-cited polyclona... Charles Farnsworth, PhD Aug 9, 2023. A Quick Guide to Neuronal and Glial Cell Type Markers. The central nervous system (CNS) is composed of highly specialized cells, each of which play a key r...Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and … Refine Results. In the CST video library, you can watch how-to videos from the Tech Tips series to improve your technique, research webinars presented by scientists from CST and other institutions, and videos relevant for specific applications or disease research areas. Receptors are the main switches initiating downstream short-range and long-distance signaling in the cell and modulating cellular activities (Czyzewicz et al. …Monoclonal Antibody for studying H3 (Ser10) phosphate. Cited in 372 publications. Validated for WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit Monoclonal Antibody (CST #3377) is …CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an ...Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for ... Aliquoting of 10x buffer is recommended if many small experiments are to be performed. 2. Thaw 10x buffer at 24-30°C, mixing end-over-end. 3. Dilute 10X Cell Lysis Buffer to a 1X solution using ddH2O. This product supplies enough 10X material to make 150mls of whole cell extract. 4. Chill 1X buffer on ice and add PMSF just prior to use. The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody. Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using c-Myc Antibody #9402.Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and … Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected]. As we continue to create and provide the highest quality of products and services, be part of the team that empowers scientists and clinicians with our best-in-class tools and excellent customer experiences. Engage and share your expertise with our current customers while helping drive results by converting potential clients into profitable ones.CiteAb’s 2022 analysis shows Cell Signaling Technology has maintained a leading position as the vendor with the most antibodies in the top 100. This figure now stands at 36, an …Cell Signaling Technology is a private, family-owned company that operates in the field of applied systems biology research, particularly focusing on cancer. The company's main offerings include the development and production of antibodies, which are validated for multiple applications by their team of Ph.D. level scientists.. Japanese shogun steakhouse, Sopraffina, Cheyenne eye clinic, Diamond nail salon, Namaste savannah, Our sons moving, Bigs chicken, Academy rockwall, Dream center peoria.